rhnorrin  (R&D Systems)

Journal: Human molecular genetics

Article Title: Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy.

doi: 10.1093/hmg/ddw041

Figure Lengend Snippet: Figure 4. Knockdown of RCBTB1 significantly reduced the activation of the Norrin- or Wnt3a-mediated Wnt/β-catenin pathway by reducing the nuclear accumulation of β-

Article Snippet: The cells were stimulated 24 h post-transfection with 0, 62.5 or 125 ng/ mL human recombinant Norrin (rhNorrin) or 100 ng/mL Wnt3a (R&D Systems), or by cotransfecting the indicated amount of NDP-expressing constructs with WT or mutant alleles for 16–18 h. Firefly and Renilla luciferase activity was measured using the Dual-Luciferase® Reporter Assay System (Promega) according to the manufacturer instructions.

Techniques: Knockdown, Activation Assay